Salaita Lab

“Mechanical force regulates ligand binding and function of PD-1”

Kaitao Li, Paul Cardenas-Lizana, Jintian Lyu, Anna V. Kellner, Menglan Li, Peiwen Cong, Valencia E. Watson, Zhou Yuan, Eunseon Ahn, Larissa Doudy, Zhenhai Li, Khalid Salaita, Rafi Ahmed & Cheng Zhu

Nature Communications, available online

https://doi.org/10.1038/s41467-024-52565-2

2024

“Quantifying T cell receptor mechanics at membrane junctions using DNA origami tension sensors”

Yuesong Hu, Jhordan Rogers, Yuxin Duan, Arventh Velusamy, Steven Narum, Sarah Al Abdullatif, Khalid Salaita

Nature Nanotechnology, available online

https://doi.org/10.1038/s41565-024-01723-0

2024

“Measuring Integrin Force Loading Rates Using a Two-Step DNA Tension Sensor”

J. Dale Combs, Alexander K. Foote, Hiroaki Ogasawara, Arventh Velusamy, Sk Aysha Rashid, Joseph Nicholas Mancuso, Khalid Salaita

Journal of American Chemical Society (JACS), available online

https://doi.org/10.1021/jacs.4c03629

2024

“Mechanical control of antigen detection and discrimination by T and B cell receptors”

Jhordan Rogers, Anna T. Bajur, Khalid Salaita, Katelyn M. Spillane

Biophysical Journal, available online

https://doi.org/10.1016/j.bpj.2024.05.020

2024

“Rolosense: Mechanical Detection of SARS-CoV-2 Using a DNA-Based Motor”

Selma Piranej, Luona Zhang, Alisina Bazrafshan, Mariana Marin, Gregory B. Melikian, Khalid Salaita

ACS Central Science, available online

https://doi.org/10.1021/acscentsci.4c00312

2024

“Cbl-b mitigates the responsiveness of naive CD8+ T cells that experience extensive tonic T cell receptor signaling”

Joel Eggert, Wendy M. Zinzow- Krame, Yuesong Hu, Elizabeth M. Kolawole, Yuan- Li Tsai, Arthur Weiss, Brian D. Evavold, Khalid Salaita, Christopher D. Scharer, Byron B. Au-Yeung

Science Signaling, volume 17, issue 22

https://doi.org/10.1126/scisignal.adh0439

2024

“Force-Induced Site-Specific Enzymatic Cleavage Probes Reveal That Serial Mechanical Engagement Boosts T Cell Activation”

Jhordan Rogers, Rong Ma, Alexander Foote, Yuesong Hu, and Khalid Salaita

Journal of American Chemical Society (JACS), volume 146, issue 11, pages 7233–7242

https://doi.org/10.1021/jacs.3c08137

2024

“Molecular Compressive Force Sensor for Mapping Forces at the Cell–Substrate Interface”

Sarah Al Abdullatif, Steven Narum, Yuesong Hu, Jhordan Rogers, Rachel Fitzgerald, and Khalid Salaita

Journal of American Chemical Society (JACS) , volume 146, issue 10, pages 6830–6836

https://doi.org/10.1021/jacs.3c13648

2024

“Simulated microgravity improves maturation of cardiomyocytes derived from human induced pluripotent stem cells”

Parvin Forghani, Aysha Rashid, Lawrence C. Armand, David Wolfson, Rui Liu, Hee Cheol Cho, Joshua T. Maxwell, Hanjoong Jo, Khalid Salaita & Chunhui Xu

Scientific Reports, volume 14, article 2243

https://doi.org/10.1038/s41598-024-52453-1

2024

“An Endosomal Escape Trojan Horse Platform to Improve Cytosolic Delivery of Nucleic Acids”

Steven Narum, Brendan Deal, Hiroaki Ogasawara, Joseph Nicholas Mancuso, Jiahui Zhang, and Khalid Salaita

ACS Nano, available online

https://doi.org/10.1021/acsnano.3c09027

2024

“DNA mechanocapsules for programmable piconewton responsive drug delivery”

Arventh Velusamy, Radhika Sharma, Sk Aysha Rashid, Hiroaki Ogasawara, and Khalid Salaita

Nature Communications, volume 15, article number: 704

https://doi.org/10.1038/s41467-023-44061-w

2024

“Turbo-Charged” DNA Motors with Optimized Sequence Enable Single-Molecule Nucleic Acid Sensing”

Luona Zhang, Selma Piranej, Arshiya Namazi, Steven Narum, and Khalid Salaita

Angewandte Chemie International edition, available online

https://doi.org/10.1002/anie.202316851

2024

“Nanodiscoidal Nucleic Acids for Gene Regulation”

Radhika Sharma, Steven Narum, Shuhong Liu, Yixiao Dong, Kyung In Baek, Hanjoong Jo, and Khalid Salaita

ACS Chemical Biology, available online

https://doi.org/10.1021/acschembio.3c00038

2023

“Heteromultivalency enables enhanced detection of nucleic acid mutations”

Brendan R. Deal, Rong Ma, Steven Narum, Hiroaki Ogasawara, Yuxin Duan, James T. Kindt, and Khalid Salaita

Nature Chemistry, available online

https://doi.org/10.1038/s41557-023-01345-4

2023

“Molecular mechanocytometry using tension-activated cell tagging”

Rong Ma, Sk Aysha Rashid, Arventh Velusamy, Brendan R. Deal, Wenchun Chen, Brian Petrich, Renhao Li, Khalid Salaita

Nature Methods, available online

https://doi.org/10.1038/s41592-023-02030-7

2023

“Optomechanically Actuated Hydrogel Platform for Cell Stimulation with Spatial and Temporal Resolution”

Allison N. Ramey-Ward, Yixiao Dong, Jin Yang, Hiroaki Ogasawara, Elizabeth C. Bremer-Sai, Olga Brazhkina, Christian Franck, Michael Davis, and Khalid Salaita

ACS Biomater. Sci. Eng., volume 9, issue 9, pages 5361–5375

https://doi.org/10.1021/acsbiomaterials.3c00516

2023

“Force Triggered Self-destructive Hydrogels”

Tharindu Rajasooriya, Hiroaki Ogasawara, Yixiao Dong, Joseph Nicholas Mancuso, Khalid Salaita

Advanced Materials, available online

https://doi.org/10.1002/adma.202305544

2023

“Detection of cellular traction forces via the force-triggered Cas12a-mediated catalytic cleavage of a fluorogenic reporter strand”

Yuxin Duan, Fania Szlam,  Yuesong Hu, Wenchun Chen, Renhao Li, Yonggang Ke,  Roman Sniecinski,  Khalid Salaita

Nature Biomedical Engineering, volume 7, pages 1404–1418

https://doi.org/10.1038/s41551-023-01114-1

2023

“All-Covalent Nuclease-Resistant and Hydrogel-Tethered DNA Hairpin Probes Map pN Cell Traction Forces”

Sk Aysha Rashid, Yixiao Dong, Hiroaki Ogasawara, Maia Vierengel, Mark Edoho Essien, Khalid Salaita

ACS Applied Materials & Interfaces, volume 15, issue 28, pages 33362–33372

https://doi.org/10.1021/acsami.3c04826

2023

“Polarized focal adhesion kinase activity within a focal adhesion during cell migration”

Xiaoquan Li, Joseph Dale Combs III, Khalid Salaita, Xiaokun Shu

Nature Chemical Biology, available online

https://doi.org/10.1038/s41589-023-01353-y

2023

“DNA Nanotechnology for Investigating Mechanical Signaling in the Immune System”

Yuesong Hu, Yuxin Duan, Khalid Salaita

Angewandte Chemie, available online

https://doi.org/10.1002/anie.202302967

2023

“Mechanically active integrins target lytic secretion at the immune synapse to facilitate cellular cytotoxicity”

Mitchell S. Wang, Yuesong Hu, Elisa E. Sanchez, Xihe Xie, Nathan H. Roy, Miguel de Jesus, Benjamin Y. Winer, Elizabeth A. Zale, Weiyang Jin, Chirag Sachar, Joanne H. Lee, Yeonsun Hong, Minsoo Kim, Lance C. Kam, Khalid Salaita & Morgan Huse

Nature Communications, volume 13, article number: 3222

2022

“Mechanotransduction at the Cell Surface and Methods to Study Receptor Forces”

Rong Ma, Brendan R. Deal and Khalid Salaita

Material-based Mechanobiology from Biomaterials Science Series, Royal Society of Chemistry

eISBN: 978-1-83916-185-8

2022

“Adhesive dynamics simulations of highly polyvalent DNA motors”

Aaron T. Blanchard, Selma Piranej, Victor Pan, Khalid Salaita

The Journal of Physical Chemistry B, volume 126, issue 39, pages 7495–7509

2022

“Chemical-to-mechanical molecular computation using DNA-based motors with onboard logic”

Selma Piranej, Alisina Bazrafshan, and Khalid Salaita

Nature Nanotechnology, volume 17, pages 514–523

2022

“DNA Tension Probes Show that Cardiomyocyte Maturation Is Sensitive to the Piconewton Traction Forces Transmitted by Integrins”

Sk Aysha Rashid, Aaron T. Blanchard, J. Dale Combs, Natasha Fernandez, Yixiao Dong, Hee Cheol Cho, and Khalid Salaita

ACS Nano, volume 16, issue 4, pages 5335–534

2022

“Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis”

Tomasz J. Nawara, Yancey D. Williams, Tejeshwar C. Rao, Yuesong Hu, Elizabeth Sztul, Khalid Salaita, and Alexa L. Mattheyses

Nature Communications, volume 13, article number 1732

2022

“Molecular Force Sensors”

Rachel L. Bender & Khalid Salaita

American Chemical Society (ACS In Focus)

eISBN: ‍9780841299177, DOI: 10.1021/acsinfocus.7e4008

2022

“ST6Gal-I-mediated sialylation of the epidermal growth factor receptor modulates cell mechanics and enhances invasion”

Tejeshwar C. Rao, Reena R. Beggs, Katherine E. Ankenbauer, Jihye Hwang, Victor Pui-Yan Ma, Khalid Salaita, Susan L. Bellis, and Alexa L. Mattheyses

Journal of Biological Chemistry, volume 298, issue 4, article 101726

2022

“Gene Regulation Using Nanodiscs Modified with HIF-1-α Antisense Oligonucleotides”

Radhika Sharma, Yixiao Dong, Yuesong Hu, Victor Pui-Yan Ma, and Khalid Salaita

Bioconjugate Chemistry, volume 33, issue 2, pages 279–293

2022

“The magnitude of LFA-1/ICAM-1 forces fine tune TCR-triggered T cell activation”

Victor Pui-Yan Ma, Yuesong Hu, Anna V. Kellner, Joshua M. Brockman, Arventh Velusamy, Aaron T. Blanchard, Brian D. Evavold, Ronen Alon, and Khalid Salaita

Science Advances, volume 8, issue 8

2021

“Supramolecular DNA Photonic Hydrogels for On-Demand Control of Coloration with High Spatial and Temporal Resolution”

Yixiao Dong, J. Dale Combs, Cong Cao, Eric R. Weeks, Alisina Bazrafshan, SK Aysha Rashid, and Khalid Salaita

Nano Letters, volume 21, issue 23, pages 9958–9965

2021

"Massively parallelized molecular force manipulation with on demand thermal and optical control"

Hanquan Su, Joshua Brockman, Yuxin Duan, Navoneel Sen, Hemani Chhabra, Alisina Bazrafshan, Aaron Blanchard, Travis Meyer, Brooke Andrews, Jonathan Doye, Yonggang Ke, Brian R. Dyer, and Khalid Salaita

Journal of the American Chemical Society, volume 143, issue 46, pages 19466–19473

2021

“Conditional Antisense Oligonucleotide Triggered by miRNA”

Jiahui Zhang, Radhika Sharma, Kitae Ryu, Patrick Shen, Khalid Salaita, Hanjoong Jo

ACS Chemical Biology, volume 16, issue 11, pages 2255–2267

2021

“Carfilzomib Treatment Causes Molecular and Functional Alterations of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes”

Parvin Forghani, Aysha Rashid, Fangxu Sun, Rui Liu, Dong Li, Megan R. Lee, Hyun Hwang, Joshua T. Maxwell, Anant Mandawat, Ronghu Wu, Khalid Salaita, and Chunhui Xu

Journal of the American Heart Association, 0:e022247

2021

“An Outside-In Swtich in Integrin Signaling Caused by Chemical and Mechanical Signals in Reactive Astrocytes”

Leonardo A. Pérez, Aysha Rashid, J. Dale Combs, Pascal Schneider, Andrés Rodríguez, Khalid Salaita and Lisette Leyton

Frontiers in Cell and Developmental Biology, volume 9, article 712627

2021

“Mechanically-triggered Hybridization Chain Reaction”

Yuxin Duan, Roxanne Glazier, Alisina Bazrafshan, Yuesong Hu, Sk Aysha Rashid, Brian Petrich, Yonggang Ke, and Khalid Salaita

Angewandte Chemie, 60, 19974-19981

2021

“Turn-key super-resolution mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope”

Aaron Blanchard, J. Dale Combs, Joshua Brockman, Anna Kellner, Roxanne Glazier, Hanquan Su, Rachel Bender, Alisina Bazrafshan, Wenchun Chen, M. Edward Quach, Renhao Li, Alexa Mattheyses, and Khalid Salaita

Nature Communications, volume 12, article number 4693

2021

“DNA-based microparticle tension sensors (µTS) for measuring cell mechanics in non-planar geometries and for high-throughput quantification”

Yuesong Hu, Victor Pui-Yan Ma, Rong Ma, Wenchun Chen, Yuxin Duan, Roxanne Glazier, Brian G. Petrich, Renhao Li, and Khalid Salaita

Angewandte Chemie, 60, 2-9

2021

“DNA Gold Nanoparticle Motors Demonstrate Processive Motion with Bursts of Speed Up to 50 nm Per Second”

Alisina Bazrafshan, Maria-Eleni Kyriazi, Brandon Alexander Holt, Wenxiao Deng, Selma Piranej, Hanquan Su, Yuesong Hu, Afaf H. El-Sagheer, Tom Brown, Gabriel A. Kwong, Antonios G. Kanaras, and Khalid Salaita

ACS Nano, vol. 15 (5), pg. 8427–8438

2021

“Smart Nucleic Acids as Future Therapeutics”

Jiahui Zhang and Khalid Salaita

Trends in Biotechnology, in press

2021

“DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells”

Rong Ma, Anna V. Kellner, Yuesong Hu, Brendan R. Deal, Aaron T. Blanchard, Khalid Salaita

Journal of Visualized Experiments, 169, e62348

2021

“Multivalent molecular tension probes as anisotropic mechanosensors: concept and simulation”

Aaron T Blanchard and Khalid Salaita

Physical Biology, vol. 18 (3), 034001

2021

“Programmable mechanically active hydrogel-based materials”

Yixiao Dong*, Allison N. Ramey-Ward*, and Khalid Salaita

Advanced Materials, 2006600

2021

“Live-cell super-resolved PAINT imaging of piconewton cellular traction forces”

Joshua M. Brockman, Hanquan Su, Aaron T. Blanchard, Yuxin Duan, Travis Meyer, M. Edward Quach, Roxanne Glazier, Alisina Bazrafshan, Rachel L. Bender, Anna V. Kellner, Hiroaki Ogasawara, Rong Ma, Florian Schueder, Brian G. Petrich, Ralf Jungmann, Renhao Li, Alexa L. Mattheyses, Yonggang Ke & Khalid Salaita

Nature Methods, volume 17, pages 1018–1024

2020

“Spectroscopic Analysis of a Library of DNA Tension Probes for Mapping Cellular Forces at Fluid Interfaces”

Roxanne Glazier, Pushkar Shinde, Hiroaki Ogasawara, Khalid Salaita

ACS Applied Materials & Interfaces, vol. 13 (2), pg. 2145–2164

2021

“Conditional Deoxyribozyme-Nanoparticle Conjugates for miRNA-Triggered Gene Regulation”

Jiahui Zhang, Rong Ma, Aaron Blanchard, Jessica Petree, Hanjoong Jo, and Khalid Salaita

ACS Applied Materials & Interfaces, vol. 12 (34), pg. 37851–37861

2020

"Mechanical stimulation of adhesion receptors using light-responsive nanoparticle actuators enhances myogenesis"

Ramey-Ward, Allison; Su, Hanquan; Salaita, Khalid

ACS Applied Materials & Interfaces, vol. 12 (32), pg. 35903–35917

2020

“Engineering DNA-Functionalized Nanostructures to Bind Nucleic Acid Targets Heteromultivalently with Enhanced Avidity”

Deal, Brendan; Ma, Rong; Ma, Victor Pui-Yan; Su, Hanquan; Kindt, James; Salaita, Khalid

Journal of the American Chemical Society, vol. 142 (21), pg. 9653–9660

2020

“Optical Control of Cytokine Signaling via Bioinspired, Polymer-Induced Latency”

Perdue, Lacey; Do, Priscilla; David, Camille; Chyong, Andrew; Kellner, Anna; Ruggieri, Amanda; Kim, Hye Ryong; Salaita, Khalid; Lesinski, Gregory; Porter, Christopher; Dreaden, Erik

Biomacromolecules, vol. 21 (7), pg. 2635–2644

2020

"EGFR activation attenuates the mechanical threshold for integrin tension and focal adhesion formation"

Tejeshwar C. Rao, Victor Pui-Yan Ma, Aaron Blanchard, Tara M. Urner, Shreya Grandhi, Khalid Salaita and Alexa L. Mattheyses

Journal of Cell Science, 133, jcs238840. doi:10.1242/jcs.238840

2020

“Tunable DNA Origami Motors Translocate Ballistically Over μm Distances at nm/s Speeds”

Alisina Bazrafshan, Travis A. Meyer, Hanquan Su, Joshua M. Brockman, Aaron, T. Blanchard, Selma Piranej, Yonggang Ke, Khalid Salaita

Angewandte Chemie, 95, 9514-9521

2020

“Variable Incidence Angle Linear Dichroism (VALiD): a technique for unique 3D orientation measurement of fluorescent ensembles”

Aaron Blanchard, Joshua Brockman, Khalid Salaita, and Alexa Mattheyses

Optics Express, Vol. 28, Issue 7, pp. 10039-10061

2020

“Forces during cellular uptake of viruses and nanoparticles at the ventral side”

Tina Wiegand, Marta Fratini, Felix Frey, Klaus Yserentant, Yang Liu, Eva Weber, Kornelia Galior, Julia Ohmes, Felix Braun, Dirk-Peter Herten, Steeve Boulant, Ulrich S. Schwarz, Khalid Salaita, E. Ada Cavalcanti-Adam & Joachim P. Spatz

Nature Communications, vol.11, Article number: 32

2020

“Super-Resolution Fluorescence Imaging Reveals That Serine Incorporator Protein 5 Inhibits Human Immunodeficiency Virus Fusion by Disrupting Envelope Glycoprotein Clusters”

Yen-Cheng Chen, Chetan Sood, Mariana Marin, Jesse Aaron, Enrico Gratton, Khalid Salaita, and Gregory B. Melikyan

ACS Nano, 14, 9, 10929-10943

2020

“Seeded Heteroepitaxial Growth of Crystallizable Collagen Triple Helices: Engineering Multifunctional Two-Dimensional Core–Shell Nanostructures”

Andrea D. Merg, Eric van Genderen, Alisina Bazrafshan, Hanquan Su, Xiaobing Zuo, Gavin Touponse, Thorsten B. Blum, Khalid Salaita, Jan Pieter Abrahams, Vincent P. Conticello

Journal of the American Chemical Society, vol. 141(51), page 20107-20117

2019

“DNA mechanotechnology reveals that integrin receptors apply pN forces in podosomes on fluid substrates”

Roxanne Glazier, Joshua Brockman, Emily Bartle, Alexa Mattheyses, Olivier Destaing & Khalid Salaita

Nature Communications, vol. 10, Article number: 4507

2019

“Emerging uses of DNA mechanical devices”

Aaron Blanchard & Khalid Salaita

Science, vol. 365, Issue 6458, pp. 1080-1081

2019

“Chameleon-Inspired Strain-Accommodating Smart Skin”

Dong, Yixiao; Bazrafshan, Alisina; Pokutta, Anastassia; Sulejmani, Fatiesa; Sun, Wei; Combs, Joseph; Clarke, Kimberly; Salaita, Khalid

ACS Nano, Vol. 13, Issue 9, pg. 9918-9926

2019

“Highly Polyvalent DNA Motors Generate 100+ pN of Force via Autochemophoresis”

Aaron Blanchard, Alisina Bazrafshan, Jacob Yi, Julia Eisman, Kevin Yehl, Teng Bian, Andrew Mugler, Khalid Salaita

Nano Letters ,Vol. 19, Issue 10, pg. 6977-6986

2019

“DNA Probes that Store Mechanical Information Reveal Transient Piconewton Forces Applied by T Cells”

Rong Ma, Anna V. Kellner, Victor Pui-Yan Ma, Hanquan Su, Brendan Reid Deal, Khalid Salaita

Proceedings of the National Academy of Sciences, 116 (34) 16949-16954

2019

“2D Crystal Engineering of Nanosheets Assembled from Helical Peptide Building Blocks”

Dr. Andrea D. Merg, Gavin Touponse, Dr. Eric van Genderen, Dr. Xiaobing Zuo, Alisina Bazrafshan, Dr. Thorsten Blum, Spencer Hughes, Prof. Khalid Salaita, Prof. Jan Pieter Abrahams, Prof. Vincent P. Conticello

Angew. Chem. Int. Ed. (in press) doi:10.1002/anie.201906214

2019

“DNA Nanotechnology as an Emerging Tool to Study Mechanotransduction in Living Systems”

Victor Pui-Yan Ma & Khalid Salaita

Small, vol. 15, issue 26, 1900961, DOI: 10.1002/smll.201900961

2019

“Localized Nanoscale Heating Leads to Ultrafast Hydrogel Volume-Phase Transition”

Jing Zhao, Hanquan Su, Gregory E. Vansuch, Zheng Liu, Khalid Salaita*, and R. Brian Dyer*

ACS Nano, Vol. 13 (1), pg. 515-525

2019

“Mechanical Proofreading: A General Mechanism to Enhance the Fidelity of Information Transfer Between Cells”

Joshua Brockman and Khalid Salaita

Frontiers of Physics , Volume 7 (14)

2019

“Location, Location, Location: EphB4:Ephrin-B2 Signaling Depends on Its Spatial Arrangement”

Roxanne Glazier and Khalid Salaita

Biophysical Journal, Vol. 115 (5), pg 754-756

2018

“A TCR mechanotransduction signaling loop induces negative selection in the thymus”

Jinsung Hong, Chenghao Ge, Prithiviraj Jothikumar, Zhou Yuan, Baoyu Liu, Ke Bai, Kaitao Li, William Rittase, Miho Shinzawa, Yun Zhang, Amy Palin, Paul Love, Xinhua Yu, Khalid Salaita, Brian D. Evavold, Alfred Singer & Cheng Zhu

Nature Immunology, vol. 19, pg. 1379–1390

2018

"Programmable Multivalent DNA-origami Tension Probes for Reporting Cellular Traction Forces"

Palash K. Dutta‡, Yun Zhang‡, Aaron Blanchard‡, Chenghao Ge, Muaz Rushdi, Kristin Weiss, Cheng Zhu, Yonggang Ke, and Khalid Salaita

Nano Letters, 18 (8), pp 4803–4811

2018

"Light-Responsive Polymer Particles as Force Clamps for the Mechanical Unfolding of Target Molecules"

Hanquan Su, Zheng Liu, Yang Liu, Victor Pui-Yan Ma, Aaron Blanchard, Jing Zhao, Kornelia Galior, R. Brian Dyer, and Khalid Salaita*

Nano Letters, 18 (4), pp 2630–2636

2018

"Molecular Tension Probes to Investigate the Mechanopharmacology of Single Cells: A Step Towards Personalized Mechano-medicine"

Kornelia Galior, Victor Pui-Yan Ma, Yang Liu, Hanquan Su, Nusaiba Baker, Reynold, Panettieri, Jr., Cherry Wongtrakool, Khalid Salaita*

Advanced Healthcare Materials, accepted

2018

"Mapping the 3D Orientation of Piconewton Integrin Traction Forces"

Joshua M. Brockman, Aaron T. Blanchard, Victor Pui-Yan Ma, Wallace D. Derricotte, Yun Zhang, Meredith E. Fay, Wilbur A. Lam, Francesco A. Evangelista, Alexa L. Mattheyses*, Khalid Salaita*

Nature Methods, 15, 115-118

2018 

"Platelet integrins exhibit anisotropic mechanosensing and harness pN forces to mediate platelet aggregation"

Yun Zhang, Yongzhi Qiu, Aaron T Blanchard, Yuan Chang, Joshua M Brockman, Victor Pui-Yan Ma, Wilbur A Lam and Khalid Salaita*

Proceedings of the National Academy of Sciences, 115 (2), 325-330

2018   

"Site-Selective RNA Splicing Nanozyme: DNAzyme and RtcB Conjugates on a Gold Nanoparticle"

Jessica R. Petree, Kevin Yehl, Kornelia Galior, Roxanne Glazier, Brendan Deal, and Khalid Salaita*

ACS Chemical Biology, 13(1), 215-224

2017

"Molecular Tension Probes for Imaging Forces at the Cell Surface"

Yang Liu, Kornelia Galior, Victor Pui-Yan Ma, and Khalid Salaita*

Accounts of Chemical Research, 50 (12), pp 2915–2924

2017

"Supported Lipid Bilayer Platforms to Probe Cell Mechanobiology"

Roxanne Glazier and Khalid Salaita

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1859 (9), Part A, Pages 1465-1482

2017

“Image-guided genomics of phenotypically heterogeneous populations reveals vascular signaling during symbiotic collective cancer invasion”

Konen, J.; Summerbell, E.; Dwivedi, B.; Galior, K.; Hou, Y.; Rusnak, L.; Chen, A.; Saltz, J; Zhou, W.; Boise, L.; Vertino, P.; Cooper, L.; Salaita, K.; Kowalski, J.; Marcus, A.

Nature Communications, 8, 15078. PMID: 28497793

2017

Chemical & Engineering News highlight, May 16th 2016, vol. 94 (2), pg. 12, “T cells tug on antigens” 
Interview explaining work  
T cells use handshakes to sort friends from foes

“DNA-based Nanoparticle Tension Sensors Reveal that T-cell Receptors Transmit Defined pN Forces to Their Antigens for Enhanced Fidelity”

Yang Liu, Lori Blanchfield, Victor Pui-Yan Ma, Rakieb Andargachew, Kornelia Galior, Zheng Liu, Brian Evavold, Khalid Salaita

Proceedings of the National Academy of Sciences,  vol. 113 no. 20, 5610–5615. 

2016

See also Nature Methods (June 2016) highlight “PCR for cellular forces” [pdf]

“The Mechanically-induced Catalytic Amplification Reaction for Readout of Receptor-Mediated Cellular Forces”

Ma, P.-Y., Victor; Liu, Yang; Yehl, Kevin; Gailor, Kornelia; Zhang, Yun; and Salaita, Khalid

Angewandte Chemie International Edition, 55(18), 5488-5492

2016

“Ratiometric tension probes for mapping receptor forces and clustering at intermembrane junctions”

Victor Ma, Victor Pui-Yan; Liu, Yang; Blanchfield, Lori; Su, Hanquan; Evavold, Brian; and Salaita, Khalid

Nano Letters vol. 16 (7), 4552–4559

2016

[SI Movie 1] [SI Movie 2]

“A General Approach for Generating Fluorescent Probes to Visualize Piconewton Forces at the Cell Surface”

Chang, Yuan; Liu, Zheng; Zhang, Yun; Galior, Kornelia; Yang, Jeffery; Salaita, Khalid

Journal of the American Chemical Society, 138 (9), 2901-2904

2016

“Knockdown of TNF-α by DNAzyme gold nanoparticles as an anti-inflammatory therapy for myocardial infarction”

Somasuntharam, I.; Yehl, K.; Carroll, S.; Maxwell, J.; Martinez, M.; Pao-Lin Che; Brown, B.; Salaita K.

Biomaterials, 83, 12–22

2016

“Nanoscale Optomechanical Actuators for Controlling Mechanotransduction in Living Cell”

Zheng Liu; Yang Liu; Yuan Chang;  Hamid Reza Seyf; Asegun Henry; Alexa L. Mattheyses; Kevin Yehl; Yun Zhang; Zhuangqun Huang; Khalid Salaita

Nature Methods, 13, 143–146

2016

“Real-time fluorescence imaging with 20 nm axial resolution”

Daniel Stabley; Thomas Oh; Sanford M. Simon; Alexa L. Mattheyses; Khalid Salaita

Nature Communications, 6, 8307

2015

“Lighting up the Force: Investigating Mechanisms of Mechanotransduction Using Fluorescent Tension Probes”

Jurchenko, Carol; Salaita, Khalid

Molecular and Cellular Biology, in press

2015

DNA-based Digital Tension Probes Reveal Early Cell Adhesion Mechanics at the Single Molecule Level
Nature Communications, 5, 5167, 2014.
Zhang, Y.; Ge, C.; Zhu, C.; Salaita, K.

[SI movie 1] [SI movie 2] [SI movie 3] [SI movie 4].

See research feature in Nature Reviews Molecular and Cell Biology See research feature in Nature Methods See research feature in C&E News See research feature in Emory eScienceCommons

Nanoparticle Tension Probes Patterned at the Nanoscale: Impact of integrin clustering on force transmission
Nano Letters, 14(10), 5539-5546, 2014. 
Liu, Y.; Medda, R.; Liu, Z.; Galior, K.; Yehl, K.; Cavalcanti-Adam, A. E.; Spatz, J.; Salaita, K.; “”,

[SI movie 1] [SI movie 2] [SI movie 3]

Integrin-generated Forces Lead to Streptavidin-Biotin Unbinding in Cellular Adhesions
Biophysical Journal, 106(7), 1436-1446, 2014.
Jurchenko, Carol; Chang, Yuan; Narui, Yoshie; Zhang, Yun; Salaita, Khalid 

[SI movie 1] [SI movie 2].

See highlight in April 7th, 2014 issue of C&E News: http://cen.acs.org/articles/92/i14/Cellular-TugWar.html

Structurally Defined Nano-scale Sheets from Self-Assembly of Collagen-Mimetic Peptides
Journal of the American Chemical Society, 136(11), 4300-4308, 2014.
Jiang, T.; Xu, C.; Liu, Y.; Liu, Z.; Wall S. J.; Zuo, X.; Lian, T.; Salaita, K.; Ni, C.; Pochan, D.; Conticello, V., P.

Quantum dots encapsulated within phospholipid membranes: phase-dependent structure, photostability, and site-selective functionalization
Journal of the American Chemical Society, 136(5), 1992–1999, 2014.
Zheng, Weiwei; Liu, Yang; West, Ana; Schuler, Erin; Yehl, Kevin; Dyer, R.; Kindt, James; Salaita, Khalid

 [SI movie 1] 

The modulation of cardiac progenitor cell function by hydrogel-dependent Notch1 activation
Biomaterials, 35(28), 8103-8112, 2014.
Boopathy, A. V.; Che, P. L.; Somasuntharam I., Fiore V. F.; Cabigas, E. B.; Ban, K.; Brown M. E.; Narui, Y.; Barker T. H.; Yoon, Y.; Salaita, K.; García, A. J.; Davis, M. E.

Membrane Tethered Delta Activates Notch and Reveals a Role for Spatio-Mechanical Regulation of the Signaling Pathway
Biophysical Journal, 105(12), 2655-2665, 2013.
Narui, Y.; Salaita, K.

[SI movie 1] [SI movie 2]

Tension Sensing Nanoparticles for Mechano-imaging at the Living Non-living Interface
Journal of the American Chemical Society, 135(14), 5320–5323, 2013.
Liu, Y.; Yehl, K.; Narui, Y.; Salaita, K.

 [movie 1] [movie 2]

Manipulating the Lateral Diffusion of Surface-Anchored EGF Demonstrates that Receptor Clustering Modulates Phosphorylation Levels
Integrative Biology, 5, 659-668, 2013.
Stabley, D.; Retterer, S.; Marshall, S.; Salaita, K.

[movie 1] [movie 2] [movie 3]

Visualizing Mechanical Tension Across Membrane Receptors with a Fluorescent Sensor
Nature Methods, 9, 64-67, 2012.
Stabley, D.; Schumacher, C.; Marshall, S.; Salaita, K.

See interview in Nature Methods[nmeth.1816.pdf]

See also C&E news highlight: [C&ENews] [C&E News video]

[youtube]

Catalytic Deoxyribozyme-Modified Nanoparticles for RNAi-Independent Gene Regulation
ACS Nano, 6(10), 9150–9157, 2012.
Yehl, K.; Joshi, J.P.; Greene, B.L.; Dyer, R. B.; Nahta, R.; Salaita, K.

The Molecular Boat: A Hands-on Experiment to Demonstrate the Forces Applied to Self-Assembled Monolayers at Interfaces
Journal of Chemical Education, 89(12), 1547–1550, 2012.
Chan, C.; Salaita, K. 

Dip-pen Nanolithography of Optically Transparent Cationic Polymers to Manipulate Spatial Organization of Proteolipid Membranes"
Chemical Science, 3, 794-799, 2012.
Narui, Y.; Salaita, K.

21.

Investigating the Role of Receptor Organization Using Geometrically Patterned Lipid Membranes
Nature Protocols, 6 , 523-539, 2011.
Nair, P. N.; Salaita, K.; Petit, R. P.; Groves, J. T.;

[link][pdf]

20.

Roles of the cytoskeleton in regulating EphA2 signals
Communicative & Integrative Biology, 3(5), 454-457, 2010.
Salaita, K.; Groves, J.T.;

[link][pdf]

19.

Restriction of Receptor Movement Alters Cellular Response: Physical Force Sensing by EphA2
Science, 327(5971), 1380-1385, 2010.
Salaita, K.*; Nair, P.N.*; Petit, R.; Das, D.; Gray, J.W.; Groves, J.T.;

[link][pdf][Science][nature materials][Nature Reviews Molecular Cell Biology][Science Signaling]

18.

The Effects of Organic Vapor on Alkanethiol Deposition Via Dip-Pen
Nanolithography,Scanning, 32 (1); 9-14, 2010.
Salaita, K.; Amarnath, A.; Higgins, T. B.; Mirkin, C. A.;,

[link]

17.

Electrostatic Imaging of DNA Microarrays Using Charged Particles
Nature Biotechnology, 26(7); 825-830, 2008.
Clack, N. C.*; Salaita, K.*; Groves, J. T.;

[link][Chemical and Engineering News] [Nature Materials]

16.

Applications of Dip-Pen Nanolithography
Nature Nanotechnology, 2; 145-155, 2007.
Salaita, K.; Wang, Y.; Vega, R. A.; Mirkin, C. A.;

[link]

15.

DPN-Generated Nanostructures as Positive Resists For Preparing Lithographic Masters or Hole Arrays
Nano Lett, , (6)11; 2493-2498, 2006.
Salaita, K.; Lee, S.-W.; Ginger, D. S; Mirkin, C. A.;

[link]

14.

Massively Parallel Dip-Pen Nanolithography with 55,000 Two-Dimensional Arrays
Salaita, K.*; Wang, Y.*; Fragala, J.; Vega, R. A.; Liu, C.; Mirkin, C. A.;

Angew. Chem. Int. Edit., 45(43); 7220-7223. (Cover Article), 2006.

[link][Nature][Science][Chemical and Engineering News]

13.

Probing Electrochemical Potential Versus Surface Distance by Molecular Orientation
J. Phys. Chem. B., 110(37); 18151-18153 ,2006. 
Sun, P.; Zong, H.; Salaita, K.; Ketter, J. B.; Hoffman, B. M.; Mirkin, C. A.;

[link]

12.

Metallomacrocycles Incorporating Cofacially Aligned Diimide Units
Chem. Asian J., 2006, 1(5); 686-692. (Cover Article)
Khoshbin, M. S.; Ovchinnikov, M. V., Salaita, K., Mirkin, C. A., Stern, C. L., Zakharov, L. N. Rheingold, A. L.;. 

[link]

11.

Nanopatterns of Biological Molecules. In Nanobiotechnology II: Concept, Applications and Perspectives
Wiley-VCH, Weinheim, Germany, Chapter 13, 235-262, 2007.
Vega, R. A.; Salaita, K.; Kakkessery, J. J.; Mirkin, C. A.;

[link]

10.

Biologically Active Protein Nanoarrays Generated Using Parallel Dip-Pen
Lee, S. W.; Oh, B. K.; Sanedrin, R. G.; Salaita, K.; Mirkin, C. A.
Nanolithography. Adv. Mat. 2006, 18, 1133-1136.

[link]

9.

Fabrication of Colloidal Nanostructures from Nanolithographically Defined Templates
Interface, 14(4); 58-59, 2005.
Salaita, K.

8.

Sub-100nm, Centimeter-Scale, Parallel Dip-Pen
Nanolithography, Small, 1(10); 940-945, 2005.
Salaita, K.; Lee, S.-W.; Wang, X.; Huang, L.; Dellinger, T. M.; Liu, C.; Mirkin, C. A.

[link]

7.

Spontaneous “Phase Separation” of Patterned Binary Alkanethiol Mixtures
J. Am. Chem. Soc, 127(32); 11283-11287, 2005.
Salaita,K.; Amarnath, A.; Maspoch, D.; Higgins, T. B.; Mirkin, C. A.

[link]

6.

Nanoarrays of Single Virus Particles
Angew. Chem. Int. Edit. 44(37); 6013-6015, 2005.
Vega, R. A.; Maspoch D.; Salaita, K.; Mirkin C. A.

[link] 

5.

The Controlled Evolution of a Single Crystal
Science, 307; 1763-1766, 2005,.
Liu, X.; Zhang, Y.; Goswami, D. K.; Okasinski, J. S.; Salaita, K.; Sun, P.; Bedzyk, M. B.; Mirkin, C. A.

[link]

4.

Surface-Bound Porphyrazines: Controlling Reduction Potentials of Self-Assembled Monolayers Through Molecular Proximity/Orientation to a Metal Surface
J. Am. Chem. Soc., 126(50); 16653-16658, 2004.
esper, B. J.; Salaita K.; Zong, Z.; Mirkin, C. A.; Barrett, A. G.; Hoffman, B. M.

[link][Science]

3.

Size and Temperature Dependence of Surface Plasmon Absorption of Gold Nanoparticles Induced by Tris(2,2'-bipyridine)ruthenium(II)
J. Phys. Chem. B. 108(40); 15543-15551, 2004.
Xu, X.-H. N.; Huang, S.; Brownlow, W.; Salaita, K.; Jeffers, R. B.

[link]

2.

A Massively Parallel Electrochemical Approach to the Miniaturization of Organic Micro- and Nanostructures on Surfaces.
Langmuir, 20(3); 962-968, 2004. 
Zhang, Y.*;Salaita, K.*; Lim. J.-H.; Lee, K.-B.; Mirkin, C. A.

[link]

1.

Electrochemical Whittling of Organic Nanostructures
Nano Lett. 2(12);1389-1392, 2002.
Zhang, Y.; Salaita, K.; Lim, J.-H.; Mirkin, C. A.

[link]

“The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forces”

Anna V. Kellner, Rae Hunter, Priscilla Do, Joel Eggert, Maya Jaffe, Delaney K. Geitgey, Miyoung Lee, Jamie A. G. Hamilton, Anthony J. Ross, Raira S. Ank, Rachel L. Bender, Rong Ma, Christopher C. Porter, Erik C. Dreaden, Byron B. Au-Yeung, Karmella A. Haynes, Curtis J. Henry, Khalid Salaita

bioRxiv, preprint

2024

“Measuring integrin force loading rates using a two-step DNA tension sensor”

J. Dale Combs, Alexander K. Foote, Hiroaki Ogasawara, Arventh Velusamy, Sk Aysha Rashid, Joseph Nicholas Mancuso, Khalid Salaita

bioRxiv, preprint

2024

“DNA Origami Tension Sensors (DOTS) to study T cell receptor mechanics at membrane junctions”

Yuesong Hu, Yuxin Duan, Arventh Velusamy, Steven Narum, Jhordan Rogers, Khalid Salaita

bioRxiv, preprint

2022

“Rolosense: Mechanical detection of SARS-CoV-2 using a DNA-based motor”

Selma Piranej, Luona Zhang, Alisina Bazrafshan, Mariana Marin, Gregory B. Melikyan, Khalid Salaita

bioRxiv, preprint

2022

“Molecular Mechanocytometry Using Tension-activated Cell Tagging (TaCT)”

Rong Ma, Arventh Velusamy, Sk Aysha Rashid, Brendan R. Deal, Wenchun Chen, Brian Petrich, Renhao Li, Khalid Salaita

bioRxiv, preprint

2023

“Mechano-Cas12a Assisted Tension Sensor (MCATS) for Massively Amplified Cell Traction Force Measurements”

Yuxin Duan, Fania Szlam, Yuesong Hu, Wenchun Chen, Renhao Li, Yonggang Ke, Roman Sniecinski, Khalid Salaita

bioRxiv, preprint

2022

“Unbreakable DNA tension probes show that cell adhesion receptors detect the molecular force-extension curve of their ligands”

Rachel L. Bender, Hiroaki Ogasawara, Anna V. Kellner, Arventh Velusamy, and Khalid Salaita

bioRxiv, preprint

2022

“Heteromultivalency enables enhanced detection of nucleic acid mutations”

Brendan Deal, Rong Ma, Hiroaki Ogasawara, James Kindt, and Khalid Salaita

ChemRxiv, preprint

2022